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Forthcoming papers in Chromosome Research
ISSN: 0967-3849 (print version)
ISSN: 1573-6849 (electronic version) Online version available Most recent entries: 2008.08.23 Chromosome Research 2008 Scholarly Manuscript Prize |
The most recent entries @ 23rd August 2008
1. Reptillian sex determination
An XX/XY heteromorphic sex chromosome system in the Australian chelid turtle Emydura macquarii, a new piece in the puzzle of sex chromosome evolution in turtles.
By Pedro Alonzo Martinez et al. Ames Iowa USA, Canberra Australia
A successful attempt to characterize the sex chromosomes in E. macquarii, where no heteromorphic sex chromosoime system has yet been described. Representing an important step towards assessing the prevalence of micro sex chromosome systems and reconstructing the evolutionary history of sex determining mechanisms in chelid turtles, and ultimately in reptiles.
2. Bovid genomics
An extended river buffalo (Bubalus bubalis, 2n=50) cytogenetic map: assignment of 68 autosomal loci by FISH-mapping and R-banding and comparison with human chromosomes.
By G. P. Di Meo et al.
Loci mapped here correspond to loci already localized on homoeologous cattle and sheep chromosomes, further confirming the extent of chromosome homoeologies among bovids. Comparison of the locations of certain specific genes in cattle with those in humans identifies nine conserved chromosome segments and complex rearrangements differentiating river buffalo and human.
3. Avian genomics
Relationship between physical and genetic distances along the Zebra Finch Z chromosome.
By María Inés Pigozzi, Buenos Aires Argentina
Nine Z chromosome genes localised with FISH on synaptonemal complexes. Their positions correlated to those on the mitotic Z. Physical and genetic distances not linearly related along most of the chromosome, due to clustering of crossovers around the telomeres. Evidence of different recombination patterns among birds with otherwise conserved genomes.
4. Replication of polytene chromosomes
Local DNA underreplication correlates with accumulation of phosphorylated H2Av in the Drosophila melanogaster polytene chromosomes.
By E. Andreyeva et al. Novosibirsk, Ruissia and Albany NY USA
Does phosphorylated H2Av exist in polytene chromosomes? Phosphorylated H2Av mapped in polytene chromosomes of animals with different mutant backgrounds, where levels of DNA underreplication were modulated by expressing different levels of the SUUR protein. Evidence to indicate that DNA underreplication leads to DSBs that accumulate as salivary gland chromosomes endoreplicate.
5. Chromosomes in sperm
Positioning of chromosome 15, 18, X and Y centromeres in sperm cells of fertile individuals and infertile patients with increased level of aneuploidy.
By Marta Olszewska et al. Poznan, Poland
Do the intranuclear positions of centromeres in sperm of males with reproductive failures and increased level of aneuploidy differ from those found in sperm from normal males? Longitudinal and spatial localization of chromosome 15, 18, X and Y centromeres analyzed. Significant positional shifts demonstrated in 15/15, 18/18 and Y/Y disomics.
6. Fish genomics
Chromosome studies of European cyprinid fishes: interspecific homology of leuciscine cytotaxonomic marker - the largest subtelocentric chromosome pair as revealed by cross-species painting.
By Petr Ráb et al. Libechov Czech Republic, Lisboan Portugal, Vodnany, Czech Republic
Laser-microdissection used to produce a whole chromosome probe for the "leuciscine" marker chromosome of the roach. Cross-species FISH used to examine chromosomes of 14 wide-ranging European species, six Iberian endemic species and the brook minnow as a representative of a sister lineage of Leuciscinae, in search of interspecific chromosome homologies.
7. Mollusc cytogenetics
Karyology of the Antarctic chiton Nuttallochiton mirandus (Thiele, 1906) (Mollusca: Polyplacophora) with some considerations on chromosome evolution in chitons.
By Gaetano Odierna et al. Naples and Ancona Italy
Help towards the resolution of karyological and evolutionary problems in a relatively unknown but biologically interesting group of molluscs, the chitons, with special emphasis on a species that is part of the relatively sparse Antarctic fauna. Good evidence of a primitive karyotype that has been extensively modified by Robertsonian changes.
8. Moluscan genomics
Molecular and cytogenetic characterization of repetitive DNA in the Antarctic polyplacophoran Nuttallochiton mirandus.
By Maria Assunta Biscotti et al. Ancona and Naples Italy
Two highly repeated DNA identified in an Antarctic chiton. One has an inserted sequence that may originate from a transposable element. Dot blot analyses, involving several molluscan species, showed different levels of conservation of the repeated DNAs. FISH on metaphase chromosomes showed that both satellites are located at centromeric regions.
9. Bovid cytogenetics
Phylogenomic study of spiral horned antelope by cross species chromosome painting.
By Jiri Rubes et al. Brno Czech Republic, Naples Italy, Prague and Dvur Kralove Czech Republic, Stellenbosch South Africa
Analysis of chromosomal rearrangements among species of large antelope in an effort to resolve discrepancies relating to evidence from morphological, molecular and karyotypic sources within Tragelaphini. Karyotypic change detected using conventional and molecular cytogenetic techniques relying on whole chromosome and subchromosomal painting probes developed from cattle using laser microdissection technology.
10. Gymnosperm genomics
The chromosomal distribution of histone methylation marks in gymnosperms differs from that of angiosperms.
By Jörg Fuchs et al. Gatersleben, Germany
The first comparative analysis of the chromosomal distribution of histone methylation marks for two gymnosperm species. Data showing striking similarities with those for angiosperms with regard to the euchromatin-specific distribution of methylated H3K4 and the localisation within heterochromatin of H3K9me2 and H3K27me1. But differences in the euchromatin-specific distribution of H3K9me1.
11. Rodent cytogenetics
Retroelements (LINEs and SINEs) in vole genomes: differential distribution in the constitutive heterochromatin.
By M. J. Acosta et al. Jaén Spain
A look at the distribution of LINEs and SINEs in voles. Both elements are plentiful in the genomes of all species analyzed. The distribution of both retroelements follows a conserved pattern, with L1 sequences especially abundant on the sex chromosomes and SINE elements confined to autosomal and X chromosome euchromatin.
12. Plant sex chromosomes
Survey of repetitive sequences in Silene latifolia with respect to their distribution on sex chromosomes.
By Tomas Cermak et al. Brno and Ceske Budejovic Czech Republic, Zurich Switzerland
A survey of major types of transposable elements in Silene latifolia, a model species with interesting sex chromosomes. Many new data including evidence of a unique pattern of repeat distribution in the Y chromosome, where some elements have accumulated while others are absent, perhaps reflecting different forces shaping the Y.
13. Primate genomics
Refinement of macaque synteny arrangement with respect to the official rheMac2 macaque sequence assembly.
By Roberta Roberto et al. Bari Italy
Application of FISH using appropriate human BAC clones to resolve discrepancies in the genome map based on chromosome painting and radiation hybrid and linkage analysis. Incorrectly positioned centromeres and unconventionally orientated DNA segments and chromosomes identified. Confirms again the usefulness of well planned FISH cytogenetics in chromosome and gene mapping.
14. Meiosis in plant hybrids
Meiotic behavior of individual chromosomes of Festuca pratensis in tetraploid Lolium multiflorum.
By D. Kopecky et al. Olomouc Czech Republic, Riverside California USA
Why is the level of meiotic chromosome pairing so unexpectedly high in hybrids of Lolium and Festuca when their genomes are so manifestly different? An attempt to understand this odd situation by quantifying the affinity of individual chromosomes as defined by extent of meiotic pairing in sensibly selected hybrid types.
15. Anuran sex chromosomes
Diversity in the origins of sex chromosomes in anurans inferred from comparative mapping of sexual differentiation genes for three species of the Raninae and Xenopodinae
By Yoshinobu Uno et al. Sapporo, Kanagawa and Tokyo Japan
Comparative genomic hybridization for X. laevis and tropicalis and comparative mapping of 8 sexual differentiation genes for X. laevis, and tropicalis, and Rana rugosa using fluorescence in-situ hybridization. Xenopus chromosomes of with homology to the sex chromosomes of R. rugosa identified. Origin and differentiation of sex chromosomes in anurans discussed.
16. Rodent cytogenetics
Molecular cloning and characterization of the repetitive DNA sequences that comprise the constitutive heterochromatin of the A and B chromosomes of the Korean field mouse (Apodemus peninsulae, Muridae, Rodentia).
By Kazumi Matsubara et al. Sapporo, Kanagawa, Kumamoto and Tsukuba Japan
Cloning of site-specific repetitive DNA sequences from A . peninsulae focusing on heterochromatin of A and B chromosomes. Evidence that the B chromosomes are derivatives of autosomes and three families of autosomal repeats were independently amplified on the Bs. B s arose in A. peninsulae independently of other Apodemus species.
17. Telomeres and transcription
Transcription and activation under environmental stress of the complex telomeric repeats of Chironomus thummi.
By Martínez-Guitarte et al. Madrid Spain
Investigation of the transcription products of the Chironomus telomere repeats. Complex terminal DNA sequences transcribed and RNAs, ranging from one monomer to multimers of the telomeric unit, are abundant in both control and heat-shock conditions. Results discussed in the light of the recent discoveries about the transcription of mammalian telomeres.
18. Stem cell karyology
Separation and Maintenance of Normal Cells from Human Embryonic Stem Cells with Trisomy 12 Mosaicism.
By Hye Won Seol et al. Seoul and Hwasung, Korea
Chromosomal abnormalities observed in cultured stem cells. Trisomy 12 correlates with changes in stem cell colony morphology. Normal and trisomy 12 cells be separated from mosaic cultures with two types of morphology using a mechanical transfer technique that may be applicable for maintaining a normal karyotype in stem cell cultures.
19. Wheat cytogenetics
Molecular and cytogenetic characterization of a durum wheat - Aegilops speltoides chromosome translocation conferring resistance to stem rust.
By Justin D. Faris et al. Fargo North Gakoya and St. Paul Minnestota USA
Resistance in wheat to stem rust found in a line that carries a chromosome translocation. Molecular and cytogenetic characterization of this line carried out with GISH and molecular markers to trace the origin of the resistance gene and facilitate deployment of the line as a resistance source in wheat breeding.
20. Bovine cytogenetics
Chromosome evolution in the subtribe Bovina (Mammalia, Bovidae): the karyotype of the Cambodian banteng (Bos javanicus birmanicus) suggests that Robertsonian translocations are related to interspecific hybridization.
By Anne Ropiquet et al. Paris France, Matieland South Africa, Arlington, Texas USA, Brno Czech Republic
Molecular cytogenetic data for the Cambodian banteng (Bos javanicus birmanicus) 2n = 56. The karyotype differs from that of B. j. javanicus 2n= 60. Robertsonian translocations have become fixed in the common ancestor of Cambodian banteng as a consequence of hybridization with the kouprey (Bos sauveli) during the Pleistocene.
